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ATCC
enterococcus faecalis atcc 29212 sensitive e enterococcus faecalis atcc 51299 resistant Enterococcus Faecalis Atcc 29212 Sensitive E Enterococcus Faecalis Atcc 51299 Resistant, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/enterococcus faecalis atcc 29212 sensitive e enterococcus faecalis atcc 51299 resistant/product/ATCC Average 99 stars, based on 1 article reviews
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LGC Standards
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Addgene inc
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Merck KGaA
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Image Search Results
Journal: bioRxiv
Article Title: Destabilized 3’UTR ARE therapeutically degrades ERBB2 in drug-resistant ERBB2+ cancer models
doi: 10.1101/2022.08.14.503914
Figure Lengend Snippet: A. Dot blots show the phospho kinase array pattern of the BT474 clone 5 wild type, BT474 clone 5 Vector, BT474 clone 5 desARE3’UTR ERBB2-3 and BT474 clone 5 desARE3’UTR c-MYC 2-3. The dot marked in red box show unique kinases down regulated only in BT474 clone 5 desARE3’UTR ERBB2-3 and the dot marked in green boxes show unique kinases downregulated only in BT474 clone 5 desARE3’UTR c-MYC 2-3. Each kinase is spotted twice. B. The bar chart shows the intensity of the phospho-kinase array dot blot for the BT474 clone 5 wild type (black bar), BT474 clone 5 Vector (blue bar), BT474 clone 5 desARE3’UTR ERBB2-3 (red bar) and BT474 clone 5 desARE3’UTR c-MYC 2-3 (green bar). C. The bar charts show YES1 pT60 and WNK1 pT60 kinases specifically down regulated in BT474 clone 5 desARE3’UTR ERBB2-3 in red bar. Controls BT474 clone 5 wild type (black bar), BT474 clone 5 Vector (blue bar), and BT474 clone 5 desARE3’UTR c-MYC 2-3 (green bar). T-test (*** Pval=0.00413, desARE3’UTRERBB2-3 for YES and WNK1) D. Venn diagram show kinases downregulated in destabilized desARE3’UTR ERBB2-3, 30 NCI-H1975 (p=0.02) blue (from RNA Seq), and in destabilized desARE3’UTR ERBB2-3 BT474 clone 5 (red-kinase array) and the shared kinases found downregulated in destabilized ERBB2 in both resistant cell lines WNK1 and YES1 in pink. E. Western blot show YES1 and GAPDH expression across the NCI-H1975 wildtype cells, vector, cells containing constructs desARE3’UTR ERBB2-1, 3 and 30 and wild type trastuzumab treated NCI-H1975 cells (n=2). F. Bar chart show quantification of the YES1 expression normalized against GAPDH on NCI-H1975 wildtype cells, vector and cells containing constructs desARE3’UTR ERBB2-1, 3 and 30 (n=2). T-test (***Pval=0.0021, desARE3’UTRERBB2-1,3,30) G. Western blot show WNK1 and GAPDH expression across the NCI-H1975 wildtype cells, vector, cells containing constructs desARE3’UTR ERBB2-1, 3 and 30 and wild type trastuzumab treated NCI-H1975 cells. H. Western blot show YES1 and GAPDH expression across the NCI-H1975 destabilized with constructs desARE3’UTR ERBB2-3 and 30 (lane 1 and 3) and over expressed with YES1 in lane 2 and 4. I. Bar chart show the wound healing closing of NCI H1975 wildtype, vector, desARE3’UTRERBB2-1, 3,30 and desARE3’UTR ERBB2-30,30 overexpressed with YES1 from 0hr to 72hrs. At 24hrs *** p=0.00025 desARE3’UTRERBB2-3,30. At 48hrs ****p=0.000018 desARE3’UTR ERBB2-3 and at 72hrs *** p=0.0003 desARE3’UTRERBB2-3 and **p=0.002 desARE3’UTRERBB2-30.
Article Snippet:
Techniques: Plasmid Preparation, Dot Blot, RNA Sequencing, Western Blot, Expressing, Construct
Journal: bioRxiv
Article Title: Destabilized 3’UTR ARE therapeutically degrades ERBB2 in drug-resistant ERBB2+ cancer models
doi: 10.1101/2022.08.14.503914
Figure Lengend Snippet: A. Microscope images of wound healing assay of NCI H1975 WT, vector, desARE3’UTRERBB2-1, 3 and 30 and desARE3’UTRERBB2-3 and 30 overexpressed with YES1.Images were taken at 0hr, 24hr, 48hrs and 72hrs at 10x.
Article Snippet:
Techniques: Microscopy, Wound Healing Assay, Plasmid Preparation
Journal:
Article Title: Development of a PCR Assay for Rapid Detection of Enterococci
doi:
Figure Lengend Snippet: Sources of the 159 enterococcal clinical isolates used in this study
Article Snippet: These strains represent the following species: E. avium (ATCC 14025), E. casseliflavus (ATCC 25788), E. cecorum (ATCC 43198), E. dispar (ATCC 51266), E. durans (ATCC 19432),
Techniques:
Journal:
Article Title: Development of a PCR Assay for Rapid Detection of Enterococci
doi:
Figure Lengend Snippet: Specificity test performed with the Enterococcus -specific 40-cycle PCR assay and DNA from a variety of gram-positive and gram-negative bacterial species
Article Snippet: These strains represent the following species: E. avium (ATCC 14025), E. casseliflavus (ATCC 25788), E. cecorum (ATCC 43198), E. dispar (ATCC 51266), E. durans (ATCC 19432),
Techniques:
Journal:
Article Title: Development of a PCR Assay for Rapid Detection of Enterococci
doi:
Figure Lengend Snippet: Example of multiplex PCR amplifications with the Enterococcus-specific PCR assay and the internal control. PCR assays (40 cycles) were performed with 1 μl of genomic DNA (1 ng/μl) from various bacteria and 4,000 copies of linearized pSL1180 as an internal control. The internal control was not amplified when target DNA was present due to competitive inhibition by amplification of Enterococcus DNA. Lanes: 2, E. avium ATCC 14025; 3, E. casseliflavus ATCC 25788; 4, E. cecorum ATCC 43198; 5, E. durans ATCC 19432; 6, E. dispar ATCC 51266; 7, E. faecalis ATCC 19433; 8, E. faecium ATCC 19434; 9, E. flavescens ATCC 49996; 10, E. gallinarum ATCC 49573; 11, E. hirae ATCC 8043; 12, E. mundtii ATCC 43186; 13, E. pseudoavium ATCC 49372; 14, E. raffinosus ATCC 49427; 15, E. saccharolyticus ATCC 43076; 16, E. solitarius ATCC 49428; 17, E. coli ATCC 25922; 1 and 18, controls to which no DNA was added; M, 100-bp molecular size standard ladder.
Article Snippet: These strains represent the following species: E. avium (ATCC 14025), E. casseliflavus (ATCC 25788), E. cecorum (ATCC 43198), E. dispar (ATCC 51266), E. durans (ATCC 19432),
Techniques: Multiplex Assay, Amplification, Inhibition
Journal:
Article Title: Development of a PCR Assay for Rapid Detection of Enterococci
doi:
Figure Lengend Snippet: Detection limits of the Enterococcus -specific PCR assays for 14 enterococcal species
Article Snippet: These strains represent the following species: E. avium (ATCC 14025), E. casseliflavus (ATCC 25788), E. cecorum (ATCC 43198), E. dispar (ATCC 51266), E. durans (ATCC 19432),
Techniques: